Get your estradiol (E2) and total testosterone (T) tested every time. Get your blood taken as close as possible to your next scheduled injection/dose (Test your blood as far away as possible from a previous dose, right before your next scheduled dose. Preferably the day of your shot, or the day before). Your blood levels of estradiol and testosterone when taken at this time are called ‘trough levels’, because they are the levels of E2 and T that are present in your body at the lowest point in your HRT dosing regimen.
Trough levels most useful for injection monotherapy; for determining if your testosterone is adequately suppressed at the lowest point of your injection cycle.
The most essential component of transfeminine HRT is to get total testosterone (T) below 50ng/dL and estradiol (E2) above 100pg/mL.
from here: https://diyhrt.info/transfem/dosing/
Also, read here for a more in-depth thingy on blood tests: https://transfemscience.org/articles/transfem-intro/#monitoring-of-therapy