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submitted 1 day ago by amia to c/diyhrt

Hi there dear blahaj.zone lemmy instance! I'm Amia, transfem. I've been since June 2025 on Estradiol Undecylate injections, 30mg/28days which seems more than enough. Progress is also pretty visible which I'm happy about and I finally got a nice sweet GP who agreed to do tests for me, nice! Now, I asked to test for my Estrogen (thus all of them and not only estradiol), Testosterone levels and also liver functions. I know that the most important part is having estradiol above 100pg/mL and testosterone below 50ng/dL but for example, with liver functions, do I need to just see if my values are just normal, or should I look into more details? Should I order more tests next time or am I being too paranoid now? I'll get those results back in a few days, so I would really like to double-check for myself how and what I am doing and maybe hear experiences and opinions from other people about all this. Thanks in advance! *PS: First of all, I know that I can just look up the needed values online but I just want to ask for advice to make sure I'm not missing out on anything. *

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Get your estradiol (E2) and total testosterone (T) tested every time. Get your blood taken as close as possible to your next scheduled injection/dose (Test your blood as far away as possible from a previous dose, right before your next scheduled dose. Preferably the day of your shot, or the day before). Your blood levels of estradiol and testosterone when taken at this time are called ‘trough levels’, because they are the levels of E2 and T that are present in your body at the lowest point in your HRT dosing regimen.

Trough levels most useful for injection monotherapy; for determining if your testosterone is adequately suppressed at the lowest point of your injection cycle.

The most essential component of transfeminine HRT is to get total testosterone (T) below 50ng/dL and estradiol (E2) above 100pg/mL.

from here: https://diyhrt.info/transfem/dosing/

Also, read here for a more in-depth thingy on blood tests: https://transfemscience.org/articles/transfem-intro/#monitoring-of-therapy

this post was submitted on 26 Feb 2026
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