In college, my mentor in the lab I worked in had perfect blots every single time. Mine were barely readable every time even though I followed protocol exactly, and asked for the prof to watch me sometimes. The girl I mentored in the lab had perfect blots every single time from her first run.

It was disheartening, I felt like an idiot haha

at least you got a horse

We got another one for the wall of shame, grab the framing kit.

I ran two westerns the other day. One half of one of them was junk, the rest was fine. I guess the primary just didn't like that side of that membrane? Even used the same mix of secondary for it all. ¯\_(ツ)_/¯