Birds along elevation gradients serve as models for examining physiological adaptations, such as elevated hemoglobin concentrations at high altitudes due to lower oxygen availability. This study aimed to examine how various ecological traits influence the haemoglobin concentration responses of multiple bird taxa to an elevation gradient. This study aimed to examine how various ecological traits influence the haemoglobin concentration responses of multiple bird taxa to an elevation gradient.

The rate of increase with elevation varied by lifestyle: Terrestrial and perching birds exhibited a steeper increase in haemoglobin concentration with elevation, aerial birds also increased haemoglobin but at a more gradual rate. However, the remaining traits did not alter how species respond to hypoxia; for example, birds increased haemoglobin at the same rate in both the dry and wet seasons, meaning seasonal changes did not strongly impact elevation‐driven haemoglobin adjustments. Elevation is the primary driver of variation, while lifestyle influences baseline levels rather than the rate of change. Despite differences in lifestyle‐driven oxygen demands, birds exhibit a similar haemoglobin response to hypoxia at moderate elevations, where adjustments remain within physiological limits, indicating that hypoxia‐driven haemoglobin adjustments occur independently of baseline oxygen demands. These findings demonstrate how birds regulate oxygen transport relative to ecological constraints.