It's a convention set by early programming languages.

In most C-like languages, if (a = b)... is also a valid comparison. The = (assignment) operation returns the assigned value as a result, which is then converted to a boolean value by the if expression. Consider this Javascript code:

let a = b = 1

1. It first declares the b variable and assigns it the value of the expression 1, which is one.
2. It returns the result of the expression b = 1, which is the assigned value, which is 1.
3. It declares the a variable and assigns the previously returned value, which is 1.

Another example:

let a = 1
let b = 2
let c = 3
console.log(a == b) // prints "false" because the comparison is false
console.log(a = b) // prints 2 because the expression returns the value of the assignment, which is 'b', which is 2

// Using this in an 'if' statement:
if (b = c) { // the result of the assignment is 3, which is converted to a boolean true
    console.log("what")
}

You can't do the same in Python (it will fail with a syntax error), but it's better to adhere to convention because it doesn't hurt anyone, but going against it might confuse programmers who have greater experience with another language. Like I was when I switched from Pascal (which uses = for comparison and := for assignment) to C.